High‐Throughput and Industrial Methods for Mrna Expression Analysis

This chapter contains sections titled: Introduction Hybridisation-based techniques DNA microarrays OFP – Oligonucleotide fingerprinting Quantikine® mRNA assay PCR-based techniques ADGE – Amplified differential gene expression DEPD® – Digital expression pattern display FDD – Automated fluorescent mRNA differential display GeneCalling iAFLP – Introduced amplified fragment length polymorphism RAGE – Rapid analysis of gene expression READSTM – Restriction enzyme analysis of differential sequences TOGA – Total gene expression analysis Sequencing-based techniques MPSSTM – Massively parallel signature sequencing SAGE – Serial analysis of gene expression MicroSAGE MiniSAGE ORESTES – Open reading frame expressed sequence tags PCR-SAGE and SAGE-Lite SADE – SAGE adaption for downsized extracts TALEST – Tandem arrayed ligation of expressed sequence tags Summary References