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进境黄瓜种子携带番茄黑环病毒的检测与鉴定

Qin-hua GAN,Chang-fa CHEN,Ying-chao WANG,Ying JI,Xing-hai WU,Xiu-ling SHAO

Food Research and Development（2013）

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Abstract
番茄黑环病毒属于检疫性有害生物,其寄主范围广泛、危害严重.本文利用DAS-ELISA方法从意大利进境的黄瓜种子中初步筛选出疑似感染有番茄黑环病毒的样品,进而对阳性样品进行RT-PCR检测,得到723 bp特异性扩增片段;确认我们在进境的黄瓜种子中检出了检疫性有害生物番茄黑环病毒.此前该病毒未有在国内分布的报道,是系统内首次在意大利进境的黄瓜种子上截获该病毒.
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Key words
reverse transcriptase pcr,plant viruses
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