2-Keto-D-Gluconate-Yielding Membrane-Bound D-Glucose Dehydrogenase From Arthrobacter Globiformis C224: Purification And Characterization
MOLECULES(2015)
摘要
Glucose dehydrogenase (GlcDH) is the rate-limiting catalyst for microbial conversion of glucose to the important organic acid 2-ketogluconic acid (2KGlcA). In this study, a D-glucose dehydrogenase was purified from the industrial 2KGlcA producer Arthrobacter globiformis C224. After four purification steps, the GlcDH was successfully purified over 180 folds and specific activity of 88.1 U/mg. A single protein band of 87 kDa was detected by SDS-PAGE. The purified GlcDH had the broad substrate specificity with the K-m values for D-glucose, D-xylose, D-galactose and maltose of 0.21 mM, 0.34 mM, 0.46 mM and 0.59 mM, respectively. The kinetic studies proved that A. globiformis GlcDH followed the ping-pong kinetic mechanism. The GlcDH showed an optimum catalytic activity at pH 5.0 and 45cC with the stable activity at temperature of 20-40 degrees C and pH of 6.0-7.0. Organic solvents, metal ions or EDTA could significantly influence the GlcDH activity to different degrees.
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关键词
Arthrobacter globiformis,2-ketogluconic acid (2KGlcA),D-glucose dehydrogenase (GlcDH),purification,enzymatic properties
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