Stereoselective high-performance liquid chromatography and analytical method characterization of evacetrapib using a brush-type chiral stationary phase: a challenging isomeric separation requiring a unique eluent system.

•A brief history of the Whelk-O 1 chiral stationary phase is presented.•Separation of evacetrapib from three isomers and multiple synthetic impurities.•Novel eluent system employing a fine-tuned modifier and additive mixture.•Hypothesis of enantioselective evacetrapib to chiral selector interaction is proposed.