Genetic Diagnosis of Polycystic Kidney Disease Using Single Cell Fluorescent Polymerase Chain Reaction

OBJECTIVE:To explore the feasibility of using single cell fluorescent polymerase chain reaction (PCR) in the preimplantation genetic diagnosis (PGD).METHOD:Single buccal cell genetic analysis was performed with fluorescent PCR of linked microsatellite D16S423, followed by electrophoresis on ABI 3730.RESULT:The amplification success rate, allele dropout rate, and diagnostic accuracy rate of the single cell fluorescent PCR were 93.3%, 10.7%, and 89.3%, respectively.CONCLUSION:Single cell fluorescent PCR is a stable and reliable approach for the PGD.