Formulation of culture medium for superoxide dismutase production by Rhodotorula glutinis RY-06 strain with high yield of beta-carotene
2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008(2008)
摘要
This paper reported the formulation of culture medium for the production of superoxide dismutase (SOD) by Rhodotorula glutinis RY-06 strain with high yield of beta-carotene. The optimum composition of medium was as follows: sucrose as carbon source, peptone as nitrogen source, the ratio of carbon source to nitrogen source as 1:1 (w/w), 500 μmol/L of Fe3+ (using FeCl 3) as metal ion of the coenzyme. The SOD activity could reach 2.19×104 U/L in the preliminary optimized medium. The results suggested that strain RY-06 not only has high yield of beta-carotene but also can produce a mass of SOD under appropriate conditions. © 2008 IEEE.
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关键词
culture medium,superoxide dismutase,yeast,biomass,coenzyme,molecular biophysics,chemical analysis,biochemistry,pharmaceuticals,enzymes,production,metal ion,capacitive sensors,nitrogen
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