降钙素基因相关肽对早期糖尿病大鼠血管ATP敏感性钾通道的影响
Chinese Journal of Pharmacology and Toxicology(2004)
Abstract
目的研究早期糖尿病大鼠血管平滑肌ATP敏感性钾通道(KATP)的变化,进一步探讨糖尿病血管功能的改变机制.方法大鼠单次注射链佐霉素60mg·kg-1制作糖尿病模型;1周或2周后,两步酶消化法进行肠系膜动脉平滑肌细胞(MASMC)的消化分离;全细胞膜片钳制技术记录MASMC的ATP敏感性钾电流(IKATP).结果在保持电位-40mV,指令电位+50 mV时,对照组,糖尿病1周和2周组MASMC的IKATP分别为(79±6),(70±7)和(48±9)pA·pF-1,糖尿病2周组的IKATP明显低于对照组.给予降钙素基因相关肽0.01~100nmol·L-1,3个组的IKATP均浓度依赖性增加,对照组:y=118.3+2.9X,r=0.887;糖尿病1周组:y=123+4.4X,r=0.981;糖尿病2周组:y=100.2+4.6X,r=0.975;糖尿病组IKATP对降钙素基因相关肽量效反应斜率高于对照组.结论早期糖尿病血管平滑肌的基础IKATP减小,IKATP对降钙素基因相关肽的量效反应斜率增加.
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Key words
ATP-sensitive,Calcitonin gene related peptide,Diabetes,Muscle,Patch clamp technique,Potassium channels,Smooth,Vascular,Whole-cell
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