Expression in Escherichia Coli of Anti-Human Tumor Necrosis Factor Alpha Scfv under the Control of T7 Promoter.

An engineering anti-human TNF-alpha single-chain antibody (ScFv) gene was cloned into the expression vector pET15b-Etag, and expressed in E. coli BL21 (DE3) under the control of T7 promoter. By using 0.1 mmol/L IPTG induction, the amount of the ScFv expression product was more than 38% of total bacterial proteins. Most of them existed in a form of inclusion body. More than 6% of total bacterial proteins were soluable and can be detected in the part of periplasm, which can bind with rhuTNF-alpha by ELISA and Dot-blotting technique.