A new thiophenyl pyrazoline fluorescent probe for Cu2+ in aqueous solution and imaging in live cell.
Journal of fluorescence(2013)
摘要
A new thiophenyl pyrazoline probe for Cu(2+) in aqueous solution was synthesized and characterized by IR, NMR, HRMS and X-ray analysis. The probe displays remarkably high selectivity and sensitivity for Cu(2+) with a detection limit of 1.919 × 10(-7) M in aqueous solution (EtOH:HEPES = 1:1, v/v, 0.02 M, pH = 7.2). In addition, the probe is further successfully used to image Cu(2+) in living cells and the probe possesses good reversibility.
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关键词
imaging,hela cell,copper ion,fluorescent probe,pyrazoline
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