Promoting the Formation of Tetramolecular G-quadruplexes under Freezing Condition

In the study, we demonstrated that freezing was able to facilitate the short DNA strand T(4)G(4) to self-assemble into a tetramolecular G-quadruplex with a low DNA concentration in the absence of any ligand, providing a fast approach to configure intermolecular G-quadruplexes. The fast formation of intermolecular G-quadruplexes may offer a convenient tool.