Aspergillus niger DLFCC-90 rhamnoside hydrolase, a new type of flavonoid glycoside hydrolase.

Tingqiang Liu,Hongshan Yu,Chunzhi Zhang,Mingchun Lu,Yongzhe Piao,Masashi Ohba,Minqian Tang,Xiaodong Yuan,Shenghua Wei,Kan Wang,Anzhou Ma,Xue Feng,Siqing Qin,Chisato Mukai,Akira Tsuji,Fengxie Jin

APPLIED AND ENVIRONMENTAL MICROBIOLOGY（2012）

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摘要

A novel rutin-alpha-L-rhamnosidase hydrolyzing a-L-rhamnoside of rutin, naringin, and hesperidin was purified and characterized from Aspergillus niger DLFCC-90, and the gene encoding this enzyme, which is highly homologous to the alpha-amylase gene, was cloned and expressed in Pichia pastoris GS115. The novel enzyme was classified in glycoside-hydrolase (GH) family 13.

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flavonoid glycoside hydrolase

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