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乳牛衣原体性流产的病原鉴定及免疫原性研究

Chang-qing QIU,Ji-zhang ZHOU,Shu-min CHENG,Xiao-an CAO

Veterinary Science in China（2006）

Cited 6|Views4
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Abstract
对采自甘肃、陕西和宁夏奶牛场的发生不明原因流产乳牛病料进行了间接血凝试验(IHA)、PCR、病原分离鉴定和MOMP基因检测,证实病原为鹦鹉热衣原体(Chlamydia psittaci).对分离株SX5和NX进行毒力稳定性和免疫原性测定,用鸡胚连传20代,SX5和NX株的毒力比较稳定,毒力效价分别为10-11 ELD50和10-10 ELD50;用这2株菌对小鼠和牛做免疫效力试验,结果显示,免疫鼠5/5和5/5保护,对照鼠0/5保护;免疫牛3/3和3/3保护,对照牛0/3保护.表明,SX5和NX株具有良好的免疫原性,可以作为疫苗研制的候选菌株.
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Key words
pathologic identification,immunogenicity,abortion in dairy cows,Chlamydia psittaci
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