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黑武士大叶醉鱼草茎段愈伤组织的诱导及植株再生

Guizhou Agricultural Sciences(2013)

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Abstract
为了建立黑武士大叶醉鱼草的快速繁殖与遗传育种体系,以大叶醉鱼草的茎段为外植体,研究其离体培养及试管苗再生途径。结果表明:愈伤组织诱导的最佳培养基为MS+2.0mg/L 2,4-D+0.2mg/L 6-BA,诱导率为85%;愈伤组织分化的最佳培养基为MS+2.0mg/L TDZ+0.2mg/L NAA,分化率为80%,平均单芽数高于13个;最佳生根培养基为1/2MS+0.5mg/L IBA,生根率为78.7%,移栽成活率为80%。
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Key words
callus induction,Buddleja davidii,plant regeneration,stem
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