Identification of Proline-Based Diketopiperazines in Roasted Coffee.
Journal of agricultural and food chemistry(2000)
摘要
Five proline-based diketopiperazines were identified in water extracts of roasted coffee proteins and roasted coffee itself. These are cyclo(pro-ile), cyclo(pro-leu), cyclo(pro-phe), cyclo(pro-pro), and cyclo(pro-val). The isolation included gel chromatography and solvent (CHCl(3)) extraction; in the case of roasted coffee brews, polyamide column chromatography was also used. The identification was achieved by LC-ESI-MS and -MS/MS by comparison of the retention time and the fragmentation pattern with reference compounds. As a second method GC-EI-MS was used. By both methods the presence of diketopiperazines in roasted coffee was unambiguously verified.
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关键词
roasted coffee,green coffee water-soluble proteins,bitterness,diketopiperazines,LC-ESI-MS,GC-EI-MS
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