Observation on the sporulation and pathogenicity of Eimeria stiedai

Objective To isolate the pure specie of Eimeria stiedai by single oocyst separation technology and observe the sporulation process. Methods The pathogenicity of isolated specie was studied by a series of indexes such as latency period,mortality,liver index,transaminase,scores of pathological changes in artificial infected young rabbits.Results The shortest time of sporulation of oocyst was 39 hours under the temperature of 29 ℃.The latency period was 13 to 15 days and the infection rate was 20%. Conclusion The experiment results indicated that the pathogenicity of pure specie of E.stiedai was proportional to the inoculated dose of continuous proliferative oocysts in a range in the inoculatd groups.