Adventitious shoot formation from carnation stem segments: a comparison of different culture procedures
Scientia Horticulturae(1996)
Abstract
Adventitious shoot regeneration from stem expiants of carnation (Dianthus caryophyllus L., cultivar ‘White Sim’) was studied using three different culture procedures: agar-gelled medium, liquid shaken medium, and an interfacial membrane raft floating on liquid medium. In all culture procedures, expiants derived from the first upper internode exhibited higher adventitious shoot formation than those from the second internode. On agar-gelled medium, the number of regenerated shoots per expiant from the first internode increased with increasing thidiazuron (TDZ) concentration (up to 4 mg l−1) in the presence of 1 mg l−1 naphthaleneacetic acid. In the liquid shaken culture, the number of regenerated shoots peaked at 1 mg TDZ l−1. Maximum shoot regeneration on an interfacial membrane raft was obtained in the presence of 0.25 mg TDZ l−1. A comparison of the overall efficiencies of shoot regeneration for each culture procedure showed that maximum shoot regeneration on the raft was twice and three times that obtained with agar-gelled and liquid shaken media, respectively.
MoreTranslated text
Key words
Interfacial membrane raft,Liquid medium,Shoot regeneration,Agar-gelled medium
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined