In vitro cytotoxicity and oxidative damage of mesoporous Nano-SiO2 on RAW 264.7 macrophages.

[Objective] To study the in vitro cytotoxicity and oxidative damage of mesoporous nano-SiO2 on RAW264.7 macrophages.[Methods] The RAW 264.7 cells were treated with different concentrations of mesoporous nano-SiO(22.5,10,50,100 μg/mL)and saline control for 24 h.MTT assays were applied for testing macrophage cytotoxicity in vitro,which were based on mitochondrial activity evaluation.Total protein(TP),lactate dehydrogenase(LDH),nitrogen monoxide(NO),glutathion(GSH),superoxide dismutase(SOD)and malondialdehyde(MDA)in supernatant and intra-cellular fluid were determined.The morphologic change of RAW 264.7 was also observed.[Results] Compared with saline control,the viability of RAW264.7 cell was decreased significantly after exposed to mesoporous nano-SiO2.The levels of TP,LDH,NO and MDA in exposed groups of 50 μg/mL and 100 μg/mL dosage were higher,while GSH and SOD levels were lower than in the saline control group,which were in accordance with the morphologic change on RAW264.7 cell exposed to mesoporous nano-SiO2.[Conclusion] Mesoporous nano-SiO2 can induce cytotoxicity and oxidative demage on RAW264.7 macrophage.