Proinflammatory cytokines differnetially affect mucin expression in LS180 cells

inflammatory cells.In the present study, we report on several cytokines secreted in response to stimulation with lipopoysaccharides (LPS) and TGF[31, as well as lymphocyte restricted cytokines.Methods: Constitutive secretion of cytokines (IL-113, IL-6, IL-7, IL-8, IL-10, and GM-CSF) by 6 pancreatic epithelial duct cell lines (AsPC-1, BxPC-3, Capan-1, Capan-2, PANC-1, and PaCa 44) was investigated by the ELISA technique.Furthermore, we stimulated these 6 cell lines with different concentrations of LPS, TGFI31, IL-4 and IFNy.After 48 hours, the concentrations of IL-113, IL-6, IL-8, and GM-CSF were determined in supematants of cell cultures.Results: All cell lines produced TGFI31 and IL-8.No production of IL-7 and IL-10 could be found.IL-113 was secreted only by BxPC-3.Capan-1, BxPC-3, and PaCa 44 secreted IL-6.GM-CSF was secreted by Capan-1, Capan-2 and PaCa 44.After stimulation with LPS and TGFI31 the secretion of all investigated cytokines, was elevated.IL-4 stimulated secretion Of IL-8 and IFN'/ induced IL-6 secretion in a dose dependent manner.Conclusion: Pancreatic duct epithelial cells secreted TGFI31, IL-1, IL-6, IL-8, and GM-CSF.Cytokine secretion could be regulated by LPS and TGFI3.There was a selective stimulation of different cytokines using IL-4 as well as IFN'/.These results suggest epithelial cells play an important role during pancreas inflammation by influencing the cytokine network.