Synthesis, Characterization and DNA-binding Studies of Chiral Ruthenium(ii) Complexes with 2-(5-Nitrofuran-2-yl)-1h-imidazo[4,5-f][1,10]phenanthroline

Two new chiral ruthenium(II) complexes Δ-[Ru(bpy)2(nfip)]2+ and Λ-[Ru(bpy)2(nfip)]2+ (in which bpy=2,2′-bipyridine, nfip=2-(5-nitrofuran-2-yl)-1H-imidazo[4,5-f][1,10]phenanthroline) have been synthesized and characterized by elemental analysis, ES-MS, 1H NMR and CD spectra. The binding properties of two chiral complexes with calf thymus DNA have been investigated by different spectrophotometric methods and viscosity measurements. A subtle but detectable difference was observed in the interaction of both enantiomer with CT-DNA, Δ enantiomer binds with DNA more strongly than Λ enantiomer. Viscosity experiments provided evidence that both Δ-[Ru(bpy)2(nfip)]2+ and Λ-[Ru(bpy)2(nfip)]2+ bound to DNA by intercalation.