The Research of Electroporation Transfect Restructured Baculovirus Genome into Insects Cells

The basic ion liposome,such as Cellfectin,is usually used to transfect cell at present.The short-age of this method is expensive and instable.While the literature report that transfect exogenous gene into insects cells by electroporation is very rare.Construct the restructured baculovirus genome GFP/BAC and transfect into the insects cells Sf9 by electroporation and liposome Cellfectin,then compare the transfection efficiency of these two methods.The result show that the transfection efficiency of the two methods respec-tively is 86.14% and 86.53%,and the filial generation restructured baculovirus continue transfect Sf9 cell and the fluorescence intensity of these two methods was not obviously distinct.The transfection efficiency of electroporation is compared with liposome Cellfectin,while the advantages of electroporation is simple opera-tion,short cycle and low cost.