First Survey of Metallo-Beta-Lactamases in Clinical Isolates of Pseudomonas Aeruginosa in A German University Hospital
Antimicrobial agents and chemotherapy(2010)
摘要
A total of 489 clinical isolates of Pseudomonas aeruginosa was investigated for metallo-beta-lactamase (MBL) production. Molecular analysis detected a bla(VIM-1) gene in the chromosome of one isolate and a bla(VIM-2) gene carried on the plasmid in seven isolates. Moreover, we showed that an initial screening by combined susceptibility testing of imipenem and ceftazidime followed by a confirmatory EDTA combination disk test represents a valid alternative to the molecular investigation of MBL genes, making MBL detection possible in routine diagnostic laboratories.
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