高效液相色谱法同时测定血清中尼莫地平和尼群地平浓度
China Pharmaceuticals(2012)
Abstract
目的建立一种同时测定尼莫地平、尼群地平血清浓度的高效液相色谱法。方法以利眠宁为内标,Shim-Pack CLC-ODS C18柱(150 mm×6 mm,5μm)为色谱柱,甲醇-水(66∶34)为流动相,流速为1.2 mL/min,柱温为40℃,乙酸乙酯为提取溶剂,检测波长为237 nm,进样量为20μL。结果尼莫地平和尼群地平质量浓度在10~300 ng/mL范围内与样品和内标峰面积比值线性关系良好,最低检测质量浓度均为1 ng/mL(S/N>3),提取回收率大于62.2%,符合生物样品分析要求。结论该方法快速、灵敏、准确,适用于临床血药浓度检测。
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Key words
HPLC,nitrendipine,serum concentration,nimodipine
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