苏云金杆菌NU-1之6种培养基发酵的对比
JOURNAL OF NORTHWEST UNIVERSITY(NATURAL SCIENCE EDITION)(2005)
Abstract
目的提高苏云金杆菌NU-1制剂的毒力.方法通过苏云金杆菌NU-1在6种培养基中发酵,研究其菌体生长、pH值变化、芽孢形成、伴孢晶体率、发酵周期等.并对其晶体蛋白进行了提纯和测定.结果 5号培养基产量最高,30 ℃发酵40 h时菌数可达80.4×108个/mL,伴孢晶体率大于90%,晶体蛋白浓度达6.312 mg/mL.结论用5号培养基作的制剂毒力最强.
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Key words
Fermentation
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