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粘红酵母苯丙氨酸解氨酶合成条件优化

Si ZHANG,Limei SUN,Jiandong CUI

China Brewing（2012）

Cited 2|Views21
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Abstract
首先利用Plackett-Burman设计及最陡爬坡试验对在摇瓶中对粘红酵母合成苯丙氨酸解氨酶的培养基和培养条件进行优化筛选,然后通过单因子试验确定最适诱导物.在此基础上,进行发酵罐葡萄糖浓度、产酶pH值以及诱导物添加时间的优化.结果显示优化的发酵培养条件为葡萄糖1g/L,蛋白胨35g/L,NaCl 5g/L,KH2PO40.25g/L,(NH4)2HPO4 1.5g/L;接种量4％；初始pH值为5；控制产酶pH值为7；诱导物为L-苯丙氨酸,分别在发酵8h和26h时添加;在上述优化条件下,最高比酶活为40.85U/g,比未优化前提高了7.3倍.
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Key words
phenylalanine ammonia lyase,culture condition optimization,induction conditions,batch and fed batch,Rhodotorula glutinis
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