Effects of Electric Shock on Escherichia coli K-12: Purification of a 23-kDa Protein from Electric Shock-induced Fluid of E.coli.

When the cells of Escherichia coli K-12 were exposed to a field of direct electric current, a protein with a molecular mass of 23-kDa was detected in an electric shock-induced fluid. The protein was purified by DEAE-cellulose column chromatography to a homogenous state on SDS-polyacrylamide gel electrophoresis. The protein was designated ESP23, an electric shock-induced fluid protein.