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生物表面活性剂高产菌的微电极脉冲选育

Chinese Journal of Bioprocess Engineering(2008)

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Abstract
用自主研制的基于梳状交叉微电极阵列结构的细胞电操作系统,开展产表面活性剂BS-37菌株选育研究。在蜗牛酶质量分数1.5%、酶解温度33℃、酶解时间3 h、酶解pH为6的条件下,制备的BS-37菌株原生质体活性达8.9%。在0.1 mmol/L Ca2+、0.5 mmol/L Mg2+及0.8 mol/L山梨醇的缓冲液条件下,电刺激原生质体,获得1株高产菌株,其发酵液的表面张力从51.47 mN/m降低至37.59 mN/m,乳化力由62%升高至85%。
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Key words
electrostimulation,biosurfactants,protoplast,microelectrode pulse
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