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超滤法提取大蒜超氧化物歧化酶的工艺研究

Modern Chemical Industry(2009)

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Abstract
在热处理除去部分杂蛋白的基础上,利用相对分子质量为5×104的聚醚砜膜对大蒜超氧化物歧化酶(SOD)进行了分离和纯化,考察了蛋白质溶液性质(pH、离子强度)和溶液流体力学性质(流速、搅拌速率)对SOD透过率的影响。在最优分离条件下,SOD纯度可高达90.6%,比活性为712.49U/mg。
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Key words
garlic,protein fractionation,superoxide dismutase,ultrafiltration
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