微生物混合转化井冈霉素制备井冈霉烯胺
Chinese Journal of Pharmaceuticals(2011)
Abstract
建立了微生物混合转化井冈霉素制备井冈霉烯胺的方法。研究了SIPI-VA18和SIPI-V08单菌转化及两菌混合转化的特点,考察了接种量、两菌混合比例和转化温度的影响。底物井冈霉素浓度5%,菌体浓度70mg/ml,SIPI-VA18和SIPI-V08的浓度比60:10,30℃转化72h,底物降解率为100%,转化液中的井冈霉烯胺浓度为6.07 mg/ml,摩尔转化率为53%。
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Key words
validoxylamine A,validamycin A,mixed microbial conversion,valienamine
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