高效液相色谱法测定烟草中多酚类物质
Southwest China Journal of Agricultural Sciences(2013)
Abstract
对超声波提取烟草中多酚的提取条件及高效液相色谱检测烟草中多酚类物质的色谱条件进行了优化,并利用内标法对多酚中的绿原酸、莨菪亭和芸香苷进行了定量研究。结果表明,最佳提取条件为:烟草样品于50%甲醇(甲醇∶水)溶液中超声波萃取20 min。最佳色谱条件为:SunFire C18(4.6 mm×250 mm,5μm)色谱柱,柱温35℃,流速1 mL/min,进样体积10μl,甲醇/乙酸水溶液梯度洗脱,检测波长340 nm。
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Key words
Polyphenols,High Performance Liquid Chromatography(HPLC),Tobacco
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