HPLC法测定新月蕨中Triphyllin A和Eruberin B的含量
China Pharmacist(2013)
Abstract
目的:建立HPLC法测定新月蕨中Triphyllin A和Eruberin B的含量.方法:采用Kromasil 100-5 C18色谱柱(250 mm×4.6 mm,5 μm);柱温30℃;流动相:甲醇-水,梯度洗脱;流速:1.0 ml·min-1;检测波长:225 nm.结果:Triphyllin A在0.23~4.60 μg(r=0.999 95),Eruberin B在0.12~2.40 μg(r=0.999 85) 范围内与峰面积呈良好的线性关系;平均回收率分别为100.66%、101.05%,RSD分别为1.57%、1.41%(n=6).结论:该方法简便、准确,可用于测定新月蕨中Triphyllin A和Eruberin B的含量.
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Key words
Triphyllin A,Eruberin B
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