The photoaffinity probe 8-N3[α-32P]ATP labels the ATP-binding sites of rabbit neutrophil and skeletal muscle actin
FEBS Letters(1983)
Abstract
8-Azido-[α-32P]ATP (8-N3-ATP) was used as a photoaffinity label for ATP binding sites in the subcellular fractions of rabbit peritoneal neutrophils. The radioactive 8-N3-ATP was specifically incorporated into one major protein of 43 kDa. The isoelectric point, molecular mass and subcellular distribution of this labeled protein closely resemble those of the actin. 8-N3-[α-32P]ATP was further tested as a photoaffinity label for the ATP binding site in the purified rabbit skeletal muscle G-actin. The radioactive 8-N3-ATP was specifically incorporated into the actin band in SDS—polyacrylamide gel. The results indicate that 8-N3-ATP can be used as a photoaffinity label for actin.
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Key words
8-N3-ATP,Actin,Photoaffinity labeling
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