FIGURE 5 from Identification of MUC1-C as a Target for Suppressing Progression of Head and Neck Squamous Cell Carcinomas

MUC1-C/STAT1 signaling regulates activation of the SOX2 gene. A, CAL27/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were analyzed for SOX2 gene transcription (left) and mRNA (right) levels. The results (mean ± SD of four determinations) are expressed as relative levels compared with that obtained for vehicle-treated cells (assigned a value of 1). B, CAL27/CshRNA and CAL27/STAT1shRNA cells were analyzed for SOX2 gene transcription (left) and mRNA (right) levels. The results (mean ± SD of four determinations) are expressed as relative levels compared with that obtained for vehicle-treated cells (assigned a value of 1). C, Schema of the SOX2 gene with highlighting localization of the PLS region that contains STAT1 binding motifs. D, Soluble chromatin from CAL27/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days was precipitated with anti-MUC1-C and anti-STAT1. The DNA samples were amplified by qPCR with primers for the SOX2 PLS region. The results (mean ± SD of three determinations) are expressed as percentage of input DNA for each sample. E, Lysates from CAL27/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were immunoblotted with antibodies against the indicated proteins. F, Lysates from CAL27/CshRNA and CAL27/STAT1shRNA cells were immunoblotted with antibodies against the indicated proteins.