FIGURE 4 from Identification of MUC1-C as a Target for Suppressing Progression of Head and Neck Squamous Cell Carcinomas

MUC1-C/STAT1 signaling regulates ∆Np63 expression. A, CAL27/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were analyzed for ∆Np63 gene transcription (left) and mRNA (right) levels. The results (mean ± SD of four determinations) are expressed as relative levels compared with that obtained for vehicle-treated cells (assigned a value of 1). B, CAL27/CshRNA and CAL27/STAT1shRNA cells were analyzed for ∆Np63 gene transcription (left) and mRNA (right) levels. The results (mean ± SD of four determinations) are expressed as relative levels compared with that obtained for vehicle-treated cells (assigned a value of 1). C, Schema of the ∆Np63 gene with highlighting localization of the PLS region that contains potential STAT1 binding motifs. D, Soluble chromatin from CAL27/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days was precipitated with anti-MUC1-C and anti-STAT1. The DNA samples were amplified by qPCR with primers for the ∆Np63 PLS region. The results (mean ± SD of three determinations) are expressed as percentage of the input DNA for each sample. E, CAL27 cells expressing the indicated vectors were treated with vehicle or DOX for 7 days. Lysates were immunoblotted with antibodies against the indicated proteins. F, Lysates from CAL27/CshRNA and CAL27/STAT1shRNA cells were immunoblotted with antibodies against the indicated proteins.