2. PERFF-seq: HCR Flow-FISH and Polymer Disassembly Protocol v1

Tsion Abay,Robert Stickels, Meril Takizawa, Ronan Chaligne,Caleb Lareau

crossref（2024）

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摘要

This protocol can be used for performing HCR Flow-FISH followed by cell/nuclei enrichment via FACS and polymer disassembly on fixed and permeabilized cells and tissue derived nuclei. This protocol should be performed after finalizing "PERFF-seq: Cell and Nuclei Preparation" for the desired sample type.

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