FIGURE 5 from Spectral Flow Cytometry Methods and Pipelines for Comprehensive Immunoprofiling of Human Peripheral Blood and Bone Marrow

Dimensionality reduction to detect differences between PBMC donors. A–D, UMAP coordinates generated from concatenated data of live, non-red blood cell singlets and 85,000 lymphocytes (A), 100,000 CD45⁺ cells (B), 90,000 lymphocytes (C) or 5,000 monocytes (D) from each donor (n = 3). Plots on the left indicate clustering of cells in two-dimensional space; plots on the right show canonically gated major cell populations overlaid onto each UMAP. E–H, UMAP for each of 3 individual donors. T/B panel UMAPs (A and E); M/N/D panel total CD45⁺ cells UMAPs (B and F); M/N/D panel lymphocytes UMAPs (C and G); M/N/D panel monocytes UMAPs (D and H). Arrows (in E, F, G, and H) indicate examples of cell populations that differ in abundance between donor samples.