Measuring Ligand-bound Protein Complexes with Proximity Labeling: A Practical Guide

Identifying the drug-target interactome of small molecule therapeutics is essential for understanding the full pharmacological effects of a compound. These therapies often induce changes within the cellular proteome, leading to unexpected consequences such as changes in the targets complexation state or off-target interactions between the compound and additional proteins. Currently, unbiased target-ID approaches are being used to embark on this task. Here we provide an overview of the strengths and limitations of these methods, and a practical step-by-step protocol for using the BioTAC system to assist with drug target and interactome ID. Identifying the drug-target interactome of small molecule therapeutics is essential for understanding the full pharmacological effects of a compound. Here we provide an overview of the strengths and limitations of current methods to measure drug interactomes, and a practical step-by-step protocol for using the BioTAC system to assist with drug target and interactome ID. image