Effects of physical form of -lactoglobulin and calcium ingestion on GLP-1 secretion, gastric emptying and energy intake in humans: a randomised crossover trial

The aim of this study was to assess whether adding Ca2+ to aggregate or native forms of beta-lactoglobulin alters gut hormone secretion, gastric emptying rates and energy intake in healthy men and women. Fifteen healthy adults (mean +/- s d: 9M/6F, age: 24 +/- 5 years) completed four trials in a randomised, double-blind, crossover design. Participants consumed test drinks consisting of 30 g of beta-lactoglobulin in a native form with (NATIVE + MINERALS) and without (NATIVE) a Ca2+-rich mineral supplement and in an aggregated form both with (AGGREG + MINERALS) and without the mineral supplement (AGGREG). Arterialised blood was sampled for 120 min postprandially to determine gut hormone concentrations. Gastric emptying was determined using C-13-acetate and C-13-octanoate, and energy intake was assessed with an ad libitum meal at 120 min. A protein x mineral interaction effect was observed for total glucagon-like peptide-1 (GLP-1TOTAL) incremental AUC (iAUC; P < 0 center dot 01), whereby MINERALS + AGGREG increased GLP-1TOTAL iAUC to a greater extent than AGGREG (1882 +/- 603 v. 1550 +/- 456 pmol center dot l(-1 )center dot 120 min, P < 0 center dot 01), but MINERALS + NATIVE did not meaningfully alter the GLP-1 iAUC compared with NATIVE (1669 +/- 547 v. 1844 +/- 550 pmol center dot l-1 center dot 120 min, P = 0 center dot 09). A protein x minerals interaction effect was also observed for gastric emptying half-life (P < 0 center dot 01) whereby MINERALS + NATIVE increased gastric emptying half-life compared with NATIVE (83 +/- 14 v. 71 +/- 8 min, P < 0 center dot 01), whereas no meaningful differences were observed between MINERALS + AGGREG v. AGGREG (P = 0 center dot 70). These did not result in any meaningful changes in energy intake (protein x minerals interaction, P = 0 center dot 06). These data suggest that the potential for Ca2+ to stimulate GLP-1 secretion at moderate protein doses may depend on protein form. This study was registered at clinicaltrials.gov (NCT04659902).