MOF@Au NPs/aptamer fluorescent probe for the selective and sensitive detection of thiamethoxam

The luminescence performance of fluorescent reagents plays a crucial role in fluorescence analysis. Therefore, in this study, a novel bi-ligand Zn-based metal-organic framework, Au nanoparticle (NP) fluorescent material was synthesized using a hydrothermal method with Zn as the metal source. Simultaneously, a DNA aptamer was introduced as a molecular recognition element to develop a Zn-based MOF@Au NPs/DNA aptamer fluorescent probe for the ultra-trace detection of thiamethoxam residues in agricultural products. The probe captured different concentrations of the target molecule, thiamethoxam, through the DNA aptamer, causing a conformational change in the DNA aptamer and bursting the fluorescence of the probe, therefore establishing a fluorometric method for thiamethoxam detection. This method is highly sensitive due to the excellent luminescence properties of the Zn-based MOF@Au NPs, and the DNA aptamer can specifically recognize thiamethoxam, offering high selectivity. The linear range of the method was 2.5-6000 x 10-11 mol L-1, with a detection limit of 8.33 x 10-12 mol L-1. This method was applied to the determination of actual samples, such as bananas, and the spiked recovery rate was found to be in the range 84.05-109.07%. Overall, the proposed probe has high sensitivity, high selectivity, and easy operation for the detection of thiamethoxam residues in actual samples. A MOFs@Au NP combined with DNA aptamers fluorescent probe for the detection of thiamethoxam was fabricated. After capturing the target thiamethoxam using the DNA aptamer, the fluorescence intensity of the probe was quenched. The probe exhibits high sensitivity due to the strong and stable fluorescence intensity of Zn-MOF@Au NPs, and the DNA aptamer can specifically recognize thiamethoxam.image