A digital platform for One-Pot signal enhanced foodborne pathogen detection based on mesophilic argonaute-driven polydisperse microdroplet reactors and machine learning

Junping Wen, Minjie Han,Niu Feng, Guoxun Chen,Feng Jiang,Jianhan Lin,Yiping Chen

Chemical Engineering Journal（2024）

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摘要

•The next-generation digital platform based on novel Ago gene editing tools.•Ultrafast polydisperse droplet preparation system for quick digital detection.•Machine learning system based on droplet area and intensity dual parameters.•True sense one step/one pot multiplex nucleic acid detection by mesophilic Ago.•Asymmetric RPA enhances the cleavage effect of CbAgo at low temperatures.

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关键词

Asymmetric amplification,Mesophilic Clostridium butyricum Argonaute,One-pot,Polydisperse microdroplet reactors,Machine learning

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