Ultra-broadband Optical Diffraction Tomography
arxiv(2024)
摘要
Optical diffraction tomography (ODT) is a powerful non-invasive 3D imaging
technique, but its combination with broadband light sources is difficult. In
this study, we introduce ultrabroadband ODT, covering over 150 nm of visible
spectral bandwidth with a lateral spatial resolution of 150 nm. Our work
addresses a critical experimental gap by enabling the measurement of broadband
refractive index changes in 3D samples, a crucial information that is difficult
to assess with existing methodologies. We present spectrally resolved ODT
images of HeLa cells, obtained via pulse-shaping based Fourier transform
spectroscopy. We, further, conduct comparative experiments using
gold-nanoparticle incubated as well as non-incubated cells. The spectral
observations enabled by ultrabroadband ODT, combined with material-dependent
refractive index responses, allow for precise three-dimensional identification
of the nano-particles within cellular structures. Our work represents a crucial
step towards spectrally and time-resolved tomography of complex 3D structures
with implications for life and materials science applications.
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