In Vitro Evaluation of Antioxidant, Antimicrobial, Cytotoxic, Anti-Inflammatory, Anti-Aging, Enzyme Inhibition Potential, and HPLC Analysis of Traditionally Used Plant Delphinium Uncinatum Wall

Delphinium uncinatum Wall. (Ranunculaceae) is a native medicinal plant found on the sub-continent that is utilized to cure various human diseases. Using a total of three different tissue parts i.e., the stem, root, and mixed plant (leaves, stem, and root), methanolic extracts were prepared and evaluated to determine the best candidate for potential bioactivity. Phytochemical constituents in prepared extracts were quantified through high-performance liquid chromatography analysis that demonstrated the presence of four flavonoid compounds, rutoside, nicotiflorin, narcissoside, and violanthin in varying proportions. Strong antibacterial and antioxidant potency but mild α-amylase and cytotoxic potential was elucidated. Highest anti-inflammatory potential was found against PLA2 (36.6 ± 2.08) and 15-LOX (30.33 ± 1.52) followed by COX-1 (25.1 ± 2.02) and COX-2 (19.33 ± 1.52). Anti-aging potential showed strong inhibitory actions toward pentosidine advanced glycation end products (AGEs) and visperlysine AGEs; however, least inhibitory activities were observed against hyaluronidase and elastase. These findings made us aware of further detailed research to determine a novel drug.