RecJf exonuclease-catalyzed signal amplified aptasensor for sensitive detection of atrazine using Ni6MnO8@C/Au nanorods and COF@MOF nanohybrids

Atrazine (ATZ) represents an endocrine-disrupting pesticide posing significant threats to both the environment and human well-being. In this study, a novel turn-on electrochemical aptasensor was successfully developed for highly sensitive ATZ detection. It relied on Ni6MnO8@C/Au nanorods (Ni6MnO8@C/AuNR) and covalent-metal organic framework nanohybrids (MCA@Ce-MOF) with RecJf-catalyzed target recycling amplification. Notably, this marks the first instance of utilizing this strategy for ATZ detection. The substrate material, ultrathin Ni6MnO8@C/AuNR nanosheets, enhanced the specific surface area, conductivity, and biocompatibility, thereby facilitating biomolecule attachment and improving sensitivity. The scaffolds, hierarchically porous MCA@CeMOF, not only elevated the loading efficiency and surface concentration of signal molecules, but also strengthened the binding firmness of biomolecules. RecJf-catalyzed target recycling was triggered in the presence of ATZ, further magnifying electrochemical signals. Leveraging these unique attributes, the developed aptasensor exhibited exceptional ATZ detection performance with a low detection limit of 4.9 fg & sdot;mL- 1, a wide linear range of 4 x 10- 5-4 ng & sdot;mL- 1, and a satisfactory recovery of 93.3-99.6% in corn samples. With its diverse signal amplification capabilities, this aptasensor holds promise for detecting various analytes in environmental monitoring applications.