Abstract 2424: Development of radiation sensitivity evaluation platform in esophageal cancer patient derived organoids

Abstract Radiation therapy (Radiotherapy) is a highly effective treatment with wide range of cancer curing. Radiotherapy allows the cancer cells to be killed or reduced in number while protecting the majority of other organs and tissues in the body. Radiotherapy is commonly used with chemotherapy or immunotherapy or radiosensitizer drugs for enhancing the treatment efficacy. Predicting individual radiation sensitivity is very important in determining the direction of future treatment after cancer diagnosis. However, it is lack of accurate and relevant preclinical model for evaluation of radiation sensitivity.In this study, we developed the platform for radiosensitivity evaluation using the Esophageal cancer patient derived organoids. Biopsy tissues from Esophageal cancer were digested to single cells and then dispensed with ECM on pillar plate (MBD co.) to forming organoids. After organoid formation, the organoids were exposed to radiation with increasing dosage. Cell viability and radiation sensitivity were calculated by 3D Celltiter glo method and Calcein AM staining. Radiation sensitivity index was determined based on radiation response and organoid growth.We included 22 patients with ESCC treat with radiation therapy from Samsung Medical Center. After surgery, we compared radiation sensitivity index with pathological grade for sensitivity and specificity of radio sensitivity evaluation platform. The sensitivity was 75.00% (95% CI 34.91% - 86.81%) and the specificity was 71.43% (95% CI 41.90% - 91.61%). This resulted in an accuracy in this population of 72.73% (95% CI 49.78% - 89.27%).This study has a very high correlation to patient matched clinical outcomes of radiation sensitive group or non-sensitive group. This radiation sensitivity evaluation platform could contribute to precision medicine in Esophageal cancer patients and it can be helpful to make decision how to cure of cancer. Citation Format: Jung Eun Kim, Mi-Hyeon Jeong, Thunyaporn Ravangnam, Sunghun Ju, Dong Woo Lee, Bosung Ku, Dongryul Oh. Development of radiation sensitivity evaluation platform in esophageal cancer patient derived organoids [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 2424.