Intelli-OVI: A new-generation clinical tool for monitoring emerging viral infections

Abstract High transmissible viruses including SARS-CoV-2 frequently accumulate novel mutations that are detected via high-throughput sequencing. However, there is a need to develop an alternative rapid and non-expensive approach. Here we developed a novel multiplex DNA detection method Intelli-OVI for analysing existing and novel mutations of SARS-CoV-2. Intelli-OVI uses the micro-disc-based method IntelliPlex and computational algorithms of objective variant identification (OVI). IntelliPlex uses micro-discs printed with a unique pictorial pattern as a labelling conjugate for DNA probes, and OVI allows simultaneous identification of several variants using multidimensional data obtained by the IntelliPlex method. Importantly, de novo mutations can be identified by decreased signals, which prompts the need to design additional primers and probes. Thus, Intelli-OVI can be upgraded to keep up with rapidly evolving viruses. We believe that Intelli-OVI will be useful in establishing a world better equipped to tackle emerging novel pathogens.