Advanced Sperm Function Testing

We present a couple with history of complete fertilization failure, despite ICSI. Their treatment history, as well as previous semen analyses, led us to suspect a diagnosis of globozoospermia. A series of additional tests was therefore performed to further assess sperm function and characteristics, including chromatin compaction, DNA fragmentation, aneuploidy, presence of cytosolic factor, centrosome integrity, ultrastructural aspects, and the genomic profile of the male gamete. These advanced sperm function assays unanimously confirmed globozoospermia, and a subsequent ICSI cycle was performed in conjunction with our proprietary assisted gamete activation protocol, resulting in the live birth of two healthy baby girls.