Smartphone-controlled portable photoelectrochemical immunosensor for point-of-care testing of carcinoembryonic antigen
CHEMICAL ENGINEERING JOURNAL(2023)
摘要
Carcinoembryonic antigen (CEA) is expressed in many tumor cells and is an important cancer marker in human serum. To assess human health, a rapid and accurate real-time detection method should be established for the sensitive detection of CEA. In this work, real-time and on-site CEA detection was achieved by constructing sandwich-type photoelectrochemical (PEC) immunosensor and using a portable electrochemical workstation controlled by a smartphone. The employment of Zn-doped Fe2O3/CdIn2S4 (Zn-Fe2O3/CdIn2S4) is proposed for the first time as a sensing platform for PEC sensor. Due to its suitable band gap combination, a stepped electron transfer pattern provides a stable photocurrent response. Ag@CuWO4 as an antibody marker can amplify the sensor signal due to its ability to form a Z-type electron transfer mode with CdIn2S4. The Box-Behnken experi-ment was used to eliminate the interaction of experimental conditions and screen out the optimal experimental conditions for the immunosensor. In addition, the PEC immunosensor has a good linear relationship with CEA in the detection range of 100 fg/mL to 100 ng/mL, with a detection limit as low as 14.7 fg/mL (S/N = 3). The immunosensor has portable characteristics, good stability, reproducibility, specificity, and storage stability. It showed appreciable recoveries and low relative standard deviations in human serum sample analysis. This PEC assay paves a new point-of-care testing method for other cancer markers, particularly environmental contaminants.
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关键词
Photoelectrochemical,Ag@CuWO4,CEA
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