Rapid determination of histamine and tyramine content in milk by azo dye-spectroscopy

ObjectiveA rapid method for determination of histamine and tyramine in milk was designed and studied by azo dye-spectroscopy.MethodsHistamine and tyramine in samples were extracted by hydrochloric acid solution， and dyed reaction was acted with azo reagent. Spectral method was used for detection within 10-15 min. External standard method was used for qualitative and quantitative analysis.ResultsThe limits of quantification of histamine and tyramine were both 10 mg/kg， which met the minimum limit requirement of 50 mg/kg specified in the current domestic and foreign standards for histamine. The recovery rate was about 90% and the relative standard deviation was about 10%. The results met the relevant requirements of food physical and chemical analysis.ConclusionThe detection method is fast， efficient， stable， reliable and cost saving. It is suitable for the rapid detection of histamine and tyramine in milk.