E-learning module for cytopathology education based on virtual microscopy

•We designed, implemented, and tested a virtual microscopy E-learning module for cytopathology education.•Participants with different expertise levels reduced their false negative diagnosis.•Non-experienced participants also increased their recognition of preinvasive lesions.•Participants with lower initial performance from both groups particularly enhance their overall performance level.•Participants engage with the module and perceive an improvement in their skills.