Using Decellularized Magnetic Microrobots to Deliver Functional Cells for Cartilage Regeneration

The use of natural cartilage extracellular matrix (ECM) has gained widespread attention in the field of cartilage tissue engineering. However, current approaches for delivering functional scaffolds for osteoarthritis (OA) therapy rely on knee surgery, which is limited by the narrow and complex structure of the articular cavity and carries the risk of injuring surrounding tissues. This work introduces a novel cell microcarrier, magnetized cartilage ECM-derived scaffolds (M-CEDSs), which are derived from decellularized natural porcine cartilage ECM. Human bone marrow mesenchymal stem cells are selected for their therapeutic potential in OA treatments. Owing to their natural composition, M-CEDSs have a biomechanical environment similar to that of human cartilage and can efficiently load functional cells while maintaining high mobility. The cells are released spontaneously at a target location for at least 20 days. Furthermore, cell-seeded M-CEDSs show better knee joint function recovery than control groups 3 weeks after surgery in preclinical experiments, and ex vivo experiments reveal that M-CEDSs can rapidly aggregate inside tissue samples. This work demonstrates the use of decellularized microrobots for cell delivery and their in vivo therapeutic effects in preclinical tests. Magnetized cartilage extracellular matrix (ECM)-derived scaffolds are reported in this work. The cells are seeded and released from the microrobots. Through ultrasonic (US)/photoacoustic (PA) imaging, microrobot aggregation is visualized in ex vivo tissues. Finally, preclinical experiments demonstrate the in vivo therapeutical effects of microrobots in a cartilage defect rat model, recovering knee joint function from 45.12% to 57.89% at week 3.image