Antibacterial functionalization of calfskin bioremediation film

Decellularized calfskins are a well-established skin substitute that retains the dermal tissue's spatial structure, facilitating skin regeneration, and is already available in the market. However, their mechanical properties can change with degradation, leading to tearing at the suture. Moreover, decellularized calfskins do not possess inherent antimicrobial abilities, which can lead to wound infection and further injury during the healing process. With the objectives of supporting the clinical use of decellularized calfskins, minimizing the probability of decellularized calfskin fracture and damage during usage, and improving their anti-infective properties, this study utilized a post-loading method to load gentamicin sulfate onto the decellularized calfskin to functionalize it for antimicrobial purposes. In addition, the mechanical and physicochemical properties of the drug-carrying film were investigated to see if they could meet the clinical requirements. The results revealed that vancomycin sulfate could be loaded onto the decellularized calfskin without affecting collagen. The tensile strength of the drug-loaded membrane was determined to be in the range of 5.53-29.25 MPa, meeting the clinical requirements. Thermal analysis and pH analysis experiments demonstrated that the drug-loaded membrane did not undergo thermal denaturation or decomposition during skin repair and remained within the normal pH range of the skin, avoiding significant fluctuations in wound pH. This work introduces the loading of drugs onto decellularized calfskins using different drug delivery techniques and selects drug-loaded films that are more suitable for clinical applications, providing a foundation for subsequent testing of the mechanical properties of the drug-loaded films in this research.image